Fig. 4.

Deduced pattern of transgene insertion in the 4C30 genome. (A) A BLAST search of the 5’ flanking sequence of the fragment (304cb0d3-cbe9-4e75-bf24-4f9390aa8525) against the C57BL/6NJ mouse genome on the Ensembl website revealed that the transgenes were inserted in the δ-sarcoglycan (Sgcd) gene; i.e., in the first intron of the Sgcd-202 coding gene and 5’ upstream of the Sgcd-201 coding gene, both of which were encoded in the reverse strand in the 4C30 mouse genome. (B) In more detail, nearly the full length (15–3,508) followed by the first 657 bp (4–660) of the 3,508-bp transgene were inserted between nucleotides 47,908,481 and 47,917,870 on chromosome 11 (positions were based on the C57BL/6NJ genome retrieved from the Ensembl database). The 9,388-bp genome sequence from nucleotides 47,908,482 to 47,917,869 was lost at the insertion site of the 4C30 genome. The genome sequence determined in this study can be obtained from DDBJ (accession number: LC496472).