Skip to main content
. Author manuscript; available in PMC: 2021 Mar 28.
Published in final edited form as: Methods Enzymol. 2020 Mar 28;637:283–308. doi: 10.1016/bs.mie.2020.02.009

Fig. 1.

Fig. 1

miRNA Biogenesis. miRNA is transcribed into pri-mRNA, which is converted to pre-miRNA by the protein complex Drosha-Pasha. Pre-miRNA is then transported out of the nucleus via the protein exportin 5. The endoribonuclease dicer cleaves pre-miRNA to form a miRNA duplex. Then, unwinding of the two strands and asymmetric assembly of one strand into RISC (RNA-induced silencing complex) occurs. The RISC complex acts via transcriptional silencing, reduced translational efficiency, or mRNA degradation.