Table 2.

Experimental design, observed responses, and predicted values of IgY-loaded microcapsules

Run order	Independent variable	EE (%)			LC (%)			APS (μm)
		Experimental	Predicted	RD (%)	Experimental	Predicted	RD (%)	Experimental	Predicted	RD (%)
1	A −1 B 1 C 0	47.38±3.57f	46.65	1.54	22.97±1.73e	23.71	−3.22	644.00±7.07bc	630.74	2.06
2	A 1 B 0 C −1	63.83±1.31d	64.95	−1.75	16.09±0.33h	17.28	−7.40	632.00±10.13bc	606.52	4.03
3	A 0 B 0 C 0	78.67±0.77a	76.78	2.40	35.28±0.35a	34.43	2.41	578.00±38.24g	574.88	0.54
4	A 1 B 0 C 1	46.33±0.05f	45.25	2.33	25.40±0.03d	24.61	3.11	627.25±10.47bcd	606.52	3.30
5	A 0 B 0 C 0	78.89±2.94a	76.78	2.67	35.38±1.32a	34.43	2.69	589.25±18.10efg	574.88	2.44
6	A 0 B 0 C 0	73.26±3.26b	76.78	−4.80	32.85±1.46b	34.43	−4.81	568.50±45.97gh	574.88	−1.12
7	A 0 B 0 C 0	73.22±0.35b	76.78	−4.86	32.84±0.16b	34.43	−4.84	581.00±35.11fg	574.88	1.05
8	A 0 B 1 C −1	69.52±1.97c	65.43	5.88	19.27±0.55g	17.43	9.55	684.25±12.04a	689.95	−0.83
9	A 0 B 1 C 1	45.87±1.05f	45.73	0.31	32.62±0.74b	32.75	−0.40	643.00±16.43bc	634.82	1.27
10	A 0 B −1 C −1	68.81±2.77c	65.43	4.91	18.20±0.73g	18.07	0.71	586.75±17.95efg	602.01	−2.60
11	A 1 B −1 C 0	61.05±0.51de	59.45	2.62	21.17±0.18f	20.30	4.11	617.75±17.46cde	597.93	3.21
12	A −1 B −1 C 0	45.89±2.56f	46.65	−1.66	21.14±1.18f	20.30	3.97	594.75±9.54defg	597.93	−0.53
13	A −1 B 0 C 1	38.83±2.02g	32.45	16.43	29.73±1.54c	28.54	4.00	616.75±36.40cde	606.52	1.66
14	A −1 B 0 C −1	45.80±1.00f	52.15	−13.86	12.56±0.28i	13.35	−6.29	564.25±13.40gh	606.52	−7.49
15	A 0 B 0 C 0	79.84±3.59a	76.78	3.83	35.80±1.61a	34.43	3.83	543.50±15.26h	574.88	−5.77
16	A 1 B 1 C 0	57.89±0.57e	59.45	−2.69	22.74±0.22ef	23.71	−4.27	615.00±13.11cdef	630.74	−2.56
17	A 0 B −1 C 1	48.13±1.38f	45.73	4.99	23.43±0.85e	25.28	−7.90	655.75±12.26ab	657.13	−0.21

Relative deviation (RD)=(experimental value–predicted value)/experimental value×100%. Experimental data are expressed as mean±standard deviation (SD), n=3. Different superscript letters in the same column denote significant differences among experimental responses as affected by different independent variables (P<0.05). In the independent variable column, A means the alginate (ALG) concentration, B represents the chitooligosaccharide (COS) concentration, and C is immunoglobulin Y (IgY)/ALG ratio; different subscript numbers indicate different levels of concentration listed in Table 1. EE: encapsulation efficiency; LC: loading capacity; APS: average particle size