Table 4.
Microencapsulation parameters and physical properties of formulation-optimized microcapsules
| Encapsulation type | EE (%) | LC (%) | APS (μm) | Long-axis length (μm) | Short-axis length (μm) | Sphericity (%) | |
|
| |||||||
| IgY | 151.75±28.93b | ||||||
| ALG+IgY | 67.12±3.39a | 31.12±1.30a | 563.25±12.91a | 614.68±55.57a | 457.08±52.71a | 74.32±4.46a | |
| COS+ALG+IgY | 65.19±2.27a | 33.75±0.97a | 588.75±19.82a | 627.24±42.45a | 487.41±86.66a | 77.41±9.87a | |
|
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| Encapsulation type | Average weight (mg) | Density (g/cm3) | Color | ||||
|
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| L | a* | b* | W | ||||
|
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| IgY | 81.70±0.60a | 3.68±0.86a | 10.68±0.85a | 78.47±0.66a | |||
| ALG+IgY | 0.22±0.02a | 2.36±0.31a | 36.01±3.16b | 1.51±0.34b | 6.05±0.93b | 35.70±3.06b | |
| COS+ALG+IgY | 0.21±0.01a | 2.01±0.16a | 38.36±4.50b | 1.44±0.22b | 6.29±0.40b | 38.02±4.49b | |
COS+ALG+IgY and ALG+IgY represent the optimized IgY-loaded microcapsule coated with and without COS, respectively. Microcapsule formulation: ALG concentration, 1.56% (15.6 g/L); COS concentration, 0 or 0.61% (6.1 g/L); IgY/ALG ratio, 62.44% (mass ratio). Data are expressed as mean±standard deviation (SD), n=3. Different lowercase letters in the same column denote significant differences among diverse samples (P<0.05). IgY: immunoglobulin Y; ALG: alginate; COS: chitooligosaccharide; EE: encapsulation efficiency; LC: loading capacity; APS: average particle size; L: lightness; a*: redness/greenness; b*: yellowness/blueness; W: whiteness