Figure 4.
Recruitment and activation of natural killer (NK) cells in tumors. C57Bl/6 mice (n=6/group) were subcutaneously implanted with the murine thyroid cancer cell line TBP-B79 in the right flank (3×106 c/mouse) and mice were treated with BRAFi (40 mg/kg daily by oral gavage) and oHSV (5×105 pfu × 3 injections by intratumoral injection) as single agents or in combination. On treatment day 7, tumors were dissected and tumor-infiltrating lymphocytes (TILs) were analyzed via flow cytometry. (A) Treatment and collection schedule for TBP-B79 tumor model. (B) Percentage of NK cells/CD45+ cells and percentage of Ki67+, CD69+, GzmB+ NK cells in tumors. (C) Percentage of NK cells/CD45+ cells and percentage of CD69+ and GzmB+ NK cells in tumor-draining lymph nodes. Each dot represents an individual mouse. Statistical analysis: non-parametric Kruskal-Wallis test/Dunn’s multiple comparisons were performed using Prism Software (GraphPad). Comparison with p values over 0.05 are deemed not statistically significant. (NK cells: CD45+, CD3-, NK1.1+). The experiment was performed twice with similar results. BRAFi, BRAF inhibitor; oHSV, oncolytic herpes simplex virus; LN, lymph nodes.