FIGURE 4.

Activation of Glo1 and inhibition of AGE‐RAGE signalling by morroniside treatment are partially blocked by a Glo1 inhibitor (BBGCP₂). A, Glo1 activity was measured by Glo1 activity assays. B‐D, Gene and protein expression of Glo1 was determined by qRT‐PCR (B) and Western blot (C, D) at day 7 after treatment. E, Intracellular level of AGEs was assessed by ELISA. F‐H, Gene and protein expression of RAGE was determined by qRT‐PCR (F) and Western blot (G, H) at day 7. The data were confirmed by three repeated tests and are presented as the means ± SD. Mor, morroniside. HG, high glucose. BBGC, BBGCP₂. *P < .05 compared with the high glucose treatment group; **P < .01; ***P < .001. #P < .05 compared with the morroniside treatment group; ##P < .01; ###P < .001