FIGURE 5.

III‐mesenchymal stem/stromal cells (MSCs) displayed preferable immunoregulatory ability over NT‐MSCs in vitro. A and B, qRT‐PCR analysis of multiple immunoregulation‐associated cytokines (A) and IDO‐1 (B) in NT‐MSCs and III‐MSCs. C, ELISA analysis of secreted cytokines in the supernatant. D‐K, FCM and statistical analyses of the differentiated Th1 (D and E), Th2 (F and G), Th17 (H and I) and Treg (J and K) cells by coculturing CD4⁺ T cells with NT‐ or III‐MSCs. Data were shown as mean ± SEM (n = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001; NS, not significant