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. 2020 Sep 10;325:335–346. doi: 10.1016/j.jconrel.2020.06.035

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — hMSC osteogenic differentiation on PLGA/PEG scaffolds. Differentiation markers for hMSCs seeded on scaffolds in osteo-permissive (OP) and osteo-inductive media (OI). (A) Alkaline phosphatase (ALP) quantification at week one comparing blank and GET-RUNX2 MPs, with OI conditions and without hMSCs as a control. (B) OSTEOCALCIN (OCN) immunostaining (red) with DAPI nuclear staining (blue) at week 2 (scale bar is 100 μm). (C) Von kossa-stained samples at week 3 (scale bar is 100 μm). Blank MPs represents cell seeded scaffold containing blank MPs cultured in osteo-permissive (OP) media. Loaded MPs represents cell-seeded scaffolds containing P21-RUNX2-8R-loaded MPs cultured in osteo-permissive (OP) media. OI media represents cell seeded scaffolds containing blank MPs cultured in osteoinductive media (OI). ** is P < .01.