Fig. 6.

hMSCs attach and proliferate within 3D bioprinted PLGA/PEG scaffolds. (A) Schematic of hMSC-laden scaffold fabrication with PF127, sintering and PF127 removal after sintering. PLGA/PEG particles were mixed with 1% Tri-actin and Pluronic 18% solution in 1:1.5 ratio. The solution was mixed with hMSCs (1 × 107 per ml). The mixture was loaded to the printing syringe and printed at 25 °C. After sintering at 37 °C and washing in cold PBS at 4 °C, scaffolds were then cultured in media for 7 days. (B) Cell proliferation was evaluated by PrestoBlue assays at different time points for the cultured scaffolds. Error bars indicate SD (n = 3). (C, D) Toluidine blue stain for cells attached to the scaffold at day 7. (C) Cells at the scaffold surface, and (D) internal staining by breaking a scaffold strand. Scale bar is 1 mm. Data is presented as mean ± SD. * p < .01.