Fig. 7.

3D bioprinting of an L5 human adult lumber vertebra. (A) L5 human adult vertebra (25 mm height, 50 mm width) (highlighted in green) was chosen to demonstrate rapid 3D bioprinting of hMSC-laden PLGA/PEG scaffold as a personalized implant. (B) STL file of the L5 vertebra was divided into two halves for 3D bioprinting. (C) Half L5 vertebrae were fabricated in less an 4 min (240 s). (D) L5 vertebrae halves could be bonded with further PLGA/PEG paste (PLGA/PEG mixed with 1% Tri-acetin and 18% PF127 (1:1.5 ratio) and ihMSCs), sintered and cold washed to generate an hMSC-laden personalized scaffold. Scale bars are 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)