Fig. 3.

Characterisation of small RNAs secreted by Trichinella spiralis by high-throughput sequencing. (A–C) The length in nucleotides (nt) and the first nucleotide (AGC or U) of small RNA sequences in adult-secreted material either with or without exonuclease treatment compared with total worm material. (D–E) The length and first nucleotide of small RNA sequences in muscle stage larvae-secreted material either with or without exonuclease treatment compared with total worm material. (G, H) Supernatant (unencapsulated) or pellet (extracellular vesicles) after ultracentrifugation of muscle stage larvae-secreted material. All plots show the number of distinct sequences found in the high-throughput small RNA sequencing data. (I) Fraction of reads mapping to different genomic features across datasets.