| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Biotin anti-human CD335 (NKp46) antibody (clone 9E2) | BioLegend | Cat#331906; RRID: AB_1027671 |
| Biotin anti-human CD3 antibody (clone OKT3) | BioLegend | Cat# 317320; RRID: AB_10916519 |
| Biotin anti-human CD19 antibody (clone HIB19) | BioLegend | Cat# 302204; RRID: AB_314234 |
| Biotin anti-human CD20 antibody (clone 2H7) | BioLegend | Cat# 302350; RRID: AB_2565524 |
| Biotin anti-human CD56 (NCAM) antibody (clone HCD56) | BioLegend | Cat# 318320; RRID: AB_893390 |
| Biotin anti-human CD203c (E-NPP3) antibody (clone NP4D6) | BioLegend | Cat# 324604; RRID: AB_756042 |
| Biotin anti-human CD66b antibody (clone G10F5) | BioLegend | Cat# 305120; RRID: AB_2566608 |
| Brilliant Violet 421 anti-human CD16 antibody (clone 3G8) | BioLegend | Cat# 302038; RRID: AB_2561578 |
| Brilliant Violet 510 anti-human CD45RA antibody (clone HI100) | BioLegend | Cat# 304142; RRID: AB_2561947 |
| Brilliant Violet 510 anti-human HLA-DR antibody (clone L243) | BioLegend | Cat# 307646; RRID: AB_2561948 |
| Brilliant Violet 510 anti-human CD15 (SSEA-1) antibody (clone W6D3) | BioLegend | Cat# 323028; RRID: AB_2563400 |
| FITC anti-human CD163 antibody (clone GHI/61) | BioLegend | Cat# 333618; RRID: AB_2563094 |
| Brilliant Violet 711 anti-human CD163 antibody (clone GHI/61) | BioLegend | Cat# 333630; RRID: AB_2650972 |
| Brilliant Violet 421 anti-human CD163 antibody (clone GHI/61) | BioLegend | Cat# 333612; RRID:AB_2562463 |
| Brilliant Violet 785 anti-human CD14 antibody (clone M5E2) | BioLegend | Cat# 301840; RRID: AB_2563425 |
| PE/Cy7 anti-human CD14 antibody (clone HCD14) | BioLegend | Cat# 325618; RRID: AB_830691 |
| FITC anti-human CD14 antibody (clone M5E2) | BioLegend | Cat# 301804; RRID: AB_314186) |
| Brilliant Violet 785 anti-human CD3 antibody (clone UCHT1) | BioLegend | Cat# 300472; RRID: AB_2687178 |
| APC anti-human CD34 antibody (clone 561) | BioLegend | Cat# 343608; RRID: AB_2228972 |
| FITC anti-human CD34 antibody (clone 561) | BioLegend | Cat# 343604; RRID: AB_1732005 |
| PerCP/Cyanine5.5 anti-human CD303 (BDCA-2) antibody (clone 201A) | BioLegend | Cat# 354210; RRID: AB_11219604 |
| PerCP/Cyanine5.5 anti-human CD141 (Thrombomodulin) antibody (clone M80) | BioLegend | Cat# 344112; RRID: AB_2561625 |
| PerCP/Cyanine5.5 anti-human CD371 (CLEC12A) antibody (clone 50C1) | BioLegend | Cat# 353612; RRID: AB_2565544 |
| PE anti-human CD370 (CLEC9A/DNGR1) antibody (clone 8F9) | BioLegend | Cat# 353804; RRID: AB_10965546 |
| PE anti-human CD5 antibody (clone L17F12) | BioLegend | Cat# 364014; RRID: AB_2565284 |
| PE anti-human CD116 antibody (clone 4H1) | BioLegend | Cat# 305908; RRID: AB_2085686 |
| PE anti-human CD206 (MMR) antibody (clone 15-2) | BioLegend | Cat# 321106; RRID: AB_571911 |
| PE anti-human CD88 (C5aR) antibody (clone S5/1) | BioLegend | Cat# 344304; RRID: AB_2067175 |
| PE/Dazzle 594 anti-human CD123 antibody (clone 6H6) | BioLegend | Cat# 306034; RRID: AB_2566450 |
| PerCP/Cyanine5.5 anti-human CD123 antibody (clone 6H6) | BioLegend | Cat# 306016; RRID: AB_2264693 |
| FITC anti-human CD1c antibody (clone L161) | BioLegend | Cat# 331518; RRID: AB_2073403 |
| PE/Cy7 anti-human CD1c antibody (clone L161) | BioLegend | Cat# 331516; RRID: AB_2275574 |
| APC anti-human CD272 (BTLA) antibody (clone MIH26) | BioLegend | Cat# 344510; RRID: AB_10613101 |
| APC anti-human CD64 antibody (clone 10.1) | BioLegend | Cat# 305014; RRID: AB_1595428 |
| APC/Cyanine7 anti-human CD45 antibody (clone HI30) | BioLegend | Cat# 304014; RRID: AB_314402 |
| APC/Cy7 Streptavidin | BioLegend | Cat# 405208 |
| Brilliant Violet 785 anti-human CD95 (Fas) antibody (clone DX2) | BioLegend | Cat# 305646; RRID: AB_2629742 |
| Brilliant Violet 711 anti-human CD8 antibody (clone SK1) | BioLegend | Cat# 344734; RRID: AB_2565243 |
| Brilliant Violet 605 anti-human CD45 antibody (clone HI30) | BioLegend | Cat# 304042; RRID: AB_2562106 |
| Brilliant Violet 650 anti-human CD45RO antibody (clone UCHL1) | BioLegend | Cat# 304232; RRID: AB_2563462 |
| Brilliant Violet 421 anti-human CD103 (Integrin E) antibody (clone Ber-ACT8) | BioLegend | Cat# 350214; RRID: AB_2563514 |
| Brilliant Violet 785 anti-human CD103 (Integrin E) antibody (clone Ber-ACT8) | BioLegend | Cat# 350230; RRID: AB_2734364 |
| FITC anti-human CD223 (LAG-3) antibody (clone 7H2C65) | BioLegend | Cat# 369210; RRID: AB_2716129 |
| FITC anti-human CD3 antibody (clone UCHT1) | BioLegend | Cat# 300406; RRID: AB_314060 |
| PE/Cy7 anti-human CD183 (CXCR3) antibody (clone G025H7) | BioLegend | Cat# 353720; RRID: AB_11219383 |
| PE/Dazzle 594 anti-human CD28 antibody (clone CD28.2) | BioLegend | Cat# 302942; RRID: AB_2564235 |
| PE anti-human CD197 (CCR7) antibody (clone G043H7) | BioLegend | Cat# 353204; RRID: AB_10913813 |
| APC/Cyanine7 anti-human CD4 antibody (clone SK3) | BioLegend | Cat# 344616; RRID: AB_2028483 |
| Alexa Fluor® 700 anti-human CD127 (IL-7Ra) antibody (clone A019D5) | BioLegend | Cat# 351344; RRID: AB_2566200 |
| APC anti-human CD279 (PD-1) antibody (clone EH12.2H7) | BioLegend | Cat# 329908; RRID: AB_940475 |
| PE anti-human CD115 antibody (clone 9-4D2-1D4) | BioLegend | Cat# 347310; RRID: AB_2565491 |
| FITC anti-human CD116 antibody (clone 4H1) | BioLegend | Cat# 305906; RRID: AB_2085687 |
| FITC anti-human CD86 antibody (clone BU63) | BioLegend | Cat# 374204; RRID: AB_2721574 |
| Human Axl PE antibody (clone 108724) | R&D | Cat# FAB154P; no RRID |
| PerCP/Cyanine5.5 anti-mouse CD45 antibody (clone 30-F11) | BioLegend | Cat# 103132; RRID: AB_893340 |
| APC-eFluor 780 anti-human HLA-DR antibody (clone LN3) | eBioscience | Cat# 47-9956; RRID:AB_1963603 |
| PE/Cy7 anti-human CD11c antibody (clone Bu15) | Biolegend | Cat# 337216; RRID:AB_2129790 |
| FITC anti-human CD14 antibody (clone 61D3) | eBioscience | Cat# 11-0149; RRID:AB_464951 |
| PerCP-eFluor 710 anti-human CD1c antibody (clone L161) | eBioscience | Cat# 46-0015; RRID:AB_10548936 |
| PE anti-human CD304 antibody (clone AD5-17F6) | Miltenyi | Cat# 130-113-517; RRID:AB_2751124 |
| PE-Cy5 Mouse anti-Human CD1a antibody (clone HI149) | BD | Cat# 555808; RRID:AB_396142 |
| Alexa Fluor® 647 anti-human CD206 (MMR) antibody (clone 15-2) | Biolegend | Cat# 321116; RRID:AB_571881 |
| VioBlue anti-human CD141 antibody (clone AD5-14H12) | Miltenyi | Cat# 130-113-882; RRID:AB_2726374 |
| Purified anti-human CD45 antibody (clone HI30) | BioLegend | Cat# 304002; RRID: AB_314390 |
| Purified anti-human TGF 1-2-3 antibody (clone 1d11) | R&D | Cat# MAB1835; RRID:AB_357931 |
| Purified anti-human TNFα antibody (clone 1825) | R&D | Cat# MAB210; RRID:AB_2240620 |
| Purified anti-human IL-15 antibody (clone 34593) | R&D | Cat# MAB247; RRID:AB_2124578 |
| Purified anti-human Lymphotoxin α antibody (clone 5802) | R&D | Cat# MAB211; RRID:AB_2138622 |
| Purified polyclonal anti-human OX40L antibody | R&D | Cat# AF1236; RRID:AB_354686 |
| Purified polyclonal anti-human IL-33 antibody | R&D | Cat# AF3625; RRID:AB_1151900 |
| Purified anti-human TNFSF8 antibody (clone 116614) | R&D | Cat# MAB1028; RRID:AB_2303710 |
| Purified Mouse IgG1 (clone 11711) | R&D | Cat# MAB002; RRID:AB_357344 |
| Purified polyclonal Goat IgG | R&D | Cat# AB-108-C; RRID:AB_354267 |
| Purified Mouse IgG2b (clone 20116) | R&D | Cat# MAB004; RRID:AB_357346 |
| Bacterial and Virus Strains | ||
| pMX-IRES-GFP | Cell biolabs | Cat# RTV-0133 |
| pMX-huFLT3L-IRES-GFP | This paper | N/A |
| pMX-huGMCSF-IRES-GFP | This paper | N/A |
| Biological Samples | ||
| Leukocyte cones for bulk RNaseq | NHS | N/A |
| Leukocytes cones for functionnal experiments | NHS | N/A |
| umbilical cord-blood for in vitro diffentiation cells | Anthony Nolan Cell Therapy Centre | N/A |
| PBMC from whole blood for blood scRNaseq | Institut Curie Hospital (Paris, France) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Liberase TL | Sigma | Cat# 5401020001 |
| Collagenase D | Sigma | Cat# 11088866001 |
| DNase I | Sigma | Cat# 10104159001 |
| Dispase | Sigma | Cat# D4693 |
| CD34 MicroBead Kit UltraPure, human | Miltenyi | Cat# 130-100-453 |
| Accucheck counting beads | Thermo Fischer | Cat# PCB100 |
| Matrigel® | BD | Cat# 354230 |
| Poly I:C | InvivoGen | Cat# 31852-29-6 |
| R848 | InvivoGen | Cat# 144875-48-9 |
| lipopolysaccharide (LPS) | Sigma-Aldrich | Cat# L2630 |
| Cell Trace Violet (CTV) | Thermo Fischer | Cat# C34557 |
| Naive Pan T cells isolation Kit | Miltenyi | Cat# 130-097-095 |
| Cytostim | Miltenyi | Cat# 130-092-172 |
| Cytofix/ cytoperm Kit | BD Bioscience | Cat# 554714 |
| Critical Commercial Assays | ||
| Human Flt-3 Ligand/FLT3L Quantikine ELISA Kit | R&D | Cat# DFK00 |
| ELISA MAX Deluxe Set Human GM-CSF | Biolegend | Cat# 432004 |
| Human Proinflammatory Chemokine Panel (13-plex) | Biolegend | Cat# 740003 |
| Human Macrophage/Microglia Panel (13-plex) | Biolegend | Cat# 740503 |
| Deposited Data | ||
| RNaseq data raw reads and processed data | This paper | GEO: SuperSeries GSE151095 |
| Experimental Models: Cell Lines | ||
| B16_Control | This paper | N/A |
| B16_human FLT3L | This paper | N/A |
| B16_human GM-CSF | This paper | N/A |
| MS5_FLT3L | Anselmi et al., 2020 | N/A |
| MS5_GM-CSF | Anselmi et al., 2020 | N/A |
| MS5_FS12 | Anselmi et al., 2020 | N/A |
| Experimental Models: Organisms/Strains | ||
| mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jakson Laboratory | Stock#005557 |
| Software and Algorithms | ||
| Genomics Suite | Partek | N/A |
| FlowJo V10 | BD | https://www.flowjo.com |
| GraphPad Prism 8 | GraphPad Software | https://www.graphpad.com |
| R4.4 | The R Foundation | https://www.r-project.org |
| Fiji | open source | https://imagej.net/Fiji |
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