Table 3.

Design of experiments and results (R1: Resolution between DHC and HCQ)

Run	Std no	Factor 1	Factor 2	Factor 3	Response: R1
		Flow rate (mL min−1)	Temp. (°C)	MP-B (ACN) (% v/v)
2	3	1.3	20	770	1.81
1	5	1.3	20	770	1.81
9	6	1.3	30	630	1.92
3	7	1.3	20	770	1.82
7	11	1.3	30	630	1.91
8	15	1.3	30	630	1.92
15	8	1.5	25	700	1.98
14	12	1.5	25	700	1.96
13	14	1.5	25	700	1.97
11	1	1.7	30	770	1.81
4	2	1.7	20	630	1.88
6	4	1.7	20	630	1.86
12	9	1.7	30	770	1.78
10	10	1.7	30	770	1.81
5	13	1.7	20	630	1.89