Table 7.
Linearity and other validation parameters results obtained by the proposed HPLC with HCQ and related impurities
| Parameters | DHC | HCQ | HCA | HCS | DCQ |
|---|---|---|---|---|---|
| Retention time (Rt) | 9.701 | 11.644 | 16.200 | 19.629 | 36.527 |
| Relative retention time (RRt) | 0.888 | 1.00 | 1.239 | 1.319 | 2.306 |
| Relative response (RR) | 0.93 | 1.00 | 1.00 | 0.74 | 2.38 |
| Linearity | |||||
| Range (µg mL−1) | 0.201–6.034 | 0.200–6.004 | 0.500–6.004 | 0.300–5.999 | 0.302–6.032 |
| Slope | 26,427.93 | 29,236.95 | 27,598.21 | 21,329.40 | 69,212.61 |
| Intercept | − 139.06 | 528.45 | 164.11 | − 463.92 | 8672.17 |
| Correlation coefficient | 0.9999 | 0.9999 | 0.9999 | 0.9999 | 0.9999 |
| LOD (µgmL−1) | 0.067 | 0.066 | 0.166 | 0.101 | 0.101 |
| LOQ (µgmL−1) | 0.201 | 0.200 | 0.500 | 0.300 | 0.302 |
| Accuracy (% of recovery)(a) | |||||
| 50% Mean ± SD | 101.2 ± 2.35 | 101.22 ± 1.54 | 98.52 ± 0.98 | 97.25 ± 2.06 | 102.65 ± 1.61 |
| 100% Mean ± SD | 98.5 ± 1.89 | 101.25 ± 2.92 | 102.58 ± 2.55 | 102.58 ± 3.58 | 98.59 ± 2.28 |
| 150% Mean ± SD | 99.2 ± 3.6 | 98.25 ± 1.05 | 99.21 ± 1.81 | 98.25 ± 3.14 | 99.85 ± 0.68 |
| Precision (%RSD)(b) | |||||
| Repeatability | 0.62 | 0.26 | 0.89 | 0.43 | 0.91 |
| Intermediate precision | 1.25 | 0.68 | 1.05 | 1.47 | 1.68 |
(a)Average of three determinations of each concentration levels
(b)RSD of six determinations of each component