Skip to main content
. Author manuscript; available in PMC: 2020 Aug 25.
Published in final edited form as: FASEB J. 2020 Apr 12;34(6):7295–7310. doi: 10.1096/fj.201903254R

Figure 2. Administration of PCI34051 (PCI) attenuates development of renal fibrosis in obstructed kidneys.

Figure 2.

Kidneys were harvested at 7 days after various treatments as indicated. Photomicrographs illustrating Masson trichrome staining of kidney tissue (200X) (A). The Masson trichrome-positive tubulointerstitial area (blue) relative to the whole area from 6 random cortical fields was analyzed (B). Kidney tissue lysates were subjected to immunoblot analysis with antibodies against α-SMA, Collagen1, Fibronectin, and HDAC8, acetyl-Cortactin and Cortacin and tubulin. Expression levels of all those proteins were quantified by densitometry and α-SMA (D), Collagen1 (E), Fibronectin (F), HDAC8 (G) and Cortacin (I) were normalized with Tubulin, respectively. Expression levels of acetyl-Cortactin were normalized with Cortacin (H). Values are the means ± S.D (n = 6). Means with different superscript letters (a-d) are significantly different from one another (P<0.05). Scale bar: 50 μM.