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. Author manuscript; available in PMC: 2020 Aug 25.
Published in final edited form as: FASEB J. 2020 Apr 12;34(6):7295–7310. doi: 10.1096/fj.201903254R

Figure 3. PCI34051(PCI) suppresses TGFβ1-induced EMT of cultured renal epithelial cells.

Figure 3.

TKPT cells were serum starved for 24 hours and then treated with PCI (6.25 μM) for 1 hour and then incubated for an additional 24 hours in the absence or presence of TGFβ1 (5 ng/ml). Cells were stained with using an antibody against HDAC8 (green) and DAPI (blue) (X 200) (A). Cell lysates were prepared for immunoblot analysis with antibodies against α-SMA, Collagen1, Fibronectin, acetyl-Cortactin, Cortacin, HDAC8 and Tubulin (B). All those proteins were quantified by densitometry, and were normalized to Tubulin, respectively (C, D, E, F, G, H). Acetyl-Cortactin was normalized to Cortacin. The values are the means ± S.D. (n = 6). Bars with different letters (a–c) are significantly different from one another (P < 0.05). Scale bar: 50 μM.