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. Author manuscript; available in PMC: 2020 Aug 25.
Published in final edited form as: FASEB J. 2020 Apr 12;34(6):7295–7310. doi: 10.1096/fj.201903254R

Figure 8. HDAC8 inhibition preserves expression of BMP-7 and klotho in the kidney after UUO injury and cultured TKPT exposed to TGF-β1.

Figure 8.

Kidneys were collected at 7 days after UUO treatments with or without PCI34051 (PCI) (A). TKPT were serum starved for 24 hours and then treated with PCI34051 (PCI) at 6.26 μM for 1 hour (D) or transfected with HDAC8 siRNA or scrambled siRNA for 24 hours (G) followed by exposure of cells to TGFβ1 (5 ng/ml) for an additional 24 hours. Kidney (A-C) and cell lysates (D-F, G-I) were subjected to immunoblot analysis with antibodies against BMP-7, Klotho and GAPDH (A, D, G). Expression levels of all those proteins were quantified by densitometry and normalized with GAPDH (B-C, E-F. H-I). Values are the means ± S.D (n = 6). Bars with different letters (a–c) are significantly different from one another (P < 0.05).