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The sequences of the primers and probes used in detecting mtDNA
| Primer/probe | Sequence (5’-3’) |
|---|---|
| Mitochondrial D-loop | |
| Forward | GGTTCTTACTTCAGGGCCATCA |
| Reverse | GATTAGACCCGTTACCATCGAGAT |
| Probe | 6FAM-TTGGTTTATCGTCCATACGTTCCCCTTA-TAMRA |
| β-actin | |
| Forward | GGGATGTTTGCTCCAACCAA |
| Reverse | GCGCTTTTGACTCAAGGATTTAA |
| Probe | VIC-CGGTCGCCTTCACCGTTCCAGTT-TAMRA |
The words in bold are represented as probe.
