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. 2020 Aug 20;12:7501–7514. doi: 10.2147/CMAR.S255687

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© 2020 Zhang et al.

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The elevated expression of exosomal circGDI2 hampered OSCC cell proliferation, migration, invasion and glycolysis. CAL-27 and SCC-15 cells (Recipient cells) were treated with 30 µg/mL of the exosomes from pcDNA-NC- or pcDNA-circGDI2-transfected Donor cells. (A and B) MTT assay for cell proliferation. (C and D) Transwell assay for cell migration and invasion. (E–H) A corresponding assay kit for glucose consumption and lactate production. (I and J) Measurement of ECAR in treated Recipient cells. (K and L) Western blot for GLUT1 and LDHA levels in treated Recipient cells. Error bars indicated SD of triplicate experiments. *P < 0.05.

Abbreviations: pcDNA-NC-exo, exosomes derived from OSCC cells transfected with pcDNA; pcDNA-circGDI2-exo, exosomes derived from OSCC cells transfected with pcDNA-circGDI2.