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. 2020 Aug 20;12:7501–7514. doi: 10.2147/CMAR.S255687

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© 2020 Zhang et al.

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CircGDI2 acted as a sponge of miR-424-5p. (A) Schematic model of the miR-424-5p-binding sequence within circGDI2 and the mutant of the binding sequence. (B and C) The luciferase activity in CAL-27 and SCC-15 cells cotransfected with circGDI2 luciferase reporter vector (circGDI2 WT) or the site-directed mutant (circGDI2 MUT) and miR-424-5p mimic or miR-NC mimic. Blots were representative of n = 6. The expression of miR-424-5p by qRT-PCR in CAL-27 and SCC-15 cells treated with pcDNA-NC-exo or pcDNA-circGDI2-exo (D and E), in 30 pairs of OSCC tissues and matched normal tissues (F), HOK, CAL-27 and SCC-15 cells (G). Blots were representative of n = 3. (H) Correlation between circGDI2 level and miR-424-5p expression in OSCC tissues using the Spearman test. *P < 0.05.

Abbreviations: pcDNA-NC-exo, exosomes derived from OSCC cells transfected with pcDNA; pcDNA-circGDI2-exo, exosomes derived from OSCC cells transfected with pcDNA-circGDI2.