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. 2020 Aug 24;10:14089. doi: 10.1038/s41598-020-71059-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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S-layer formation during cell division. Airyscan confocal images of C. difficile 630 cells during and immediately after cell division with HADA labelled peptidoglycan cell wall (blue) and new surface SlpA_R20291 immunolabeled with Cy5 (white). Large, dark areas lacking HADA staining mark peptidoglycan synthesis at the septum between cells or a newly produced cell pole. Scale bar indicates 3 µm. On the right-hand side of each row is a schematic diagram illustrating the position of new surface SlpA_R20291 (HMW-SLP, spotted light blue and LMW-SLP, spotted orange) as detected in the corresponding microscopy images against the position of endogenous surface SlpA₆₃₀ (HMW-SLP, dark blue and LMW-SLP, red). The position of newly synthesized cell wall is displayed in brown/white stripes.