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. 2018 Sep 4;3(1):ysy016. doi: 10.1093/synbio/ysy016

Figure 1.

Figure 1.

Whey permeate enhances cell-free protein synthesis. (a) Schematic of whey permeate enhanced cell-free transcription–translation reactions (b) Endpoint (5 h) analysis of cell-free GFP production in cell-free reactions with 0–5.748 g/l whey permeate. These data were normalized to the relative fluorescence of cell-free reactions containing 0 g/l whey permeate. (c) Time-course analysis of GFP production in cell-free reactions with 0 or 0.004 g/l of whey permeate. (d) Time-course analysis of pH in cell-free reactions which included 0–5.748 g/l of whey permeate. Control reactions were whey permeate without cell extract. Error bars denote standard error of the mean. Student’s t-test, *P < 0.05, ***P < 0.0001 and ****P < 0.0001, n = 3–9.