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. 2018 Sep 4;3(1):ysy016. doi: 10.1093/synbio/ysy016

Figure 2.

Figure 2.

Cell-free characterization of phaCAB biosynthetic operons. (a) Schematic of whey permeate enhanced cell-free transcription–translation reactions for prototyping phaCAB operons. (b) Endpoint (5 h) GC-MS analysis of 3HB production in cell-free phaCAB operon prototyping reactions. (c) Time-course analysis of pH in cell-free phaCAB prototyping reactions. (d) Endpoint analysis of Acetyl-CoA content in cell-free phaCAB prototyping reactions. Error bars denote standard error of the mean. Student’s t-test, *P < 0.05, **P < 0.01 and ***P < 0.0001, n = 3–9.