Figure 2.

Cell-free protein synthesis optimization for P. putida enhances sfGFP expression yields. The CFPS reaction was optimized by surveying a range of (A) lysate content; (B) reaction temperatures; (C) plasmid concentrations; (D) magnesium concentrations. Initial reaction conditions before the optimization: 33% (v/v) lysate content, 20 ng/µl plasmid template, 12 mM magnesium concentration and 26°C. Average sfGFP yield with error bars representing standard deviations of three independent experiments is shown.