Figure 3.

sfGFP yield with various PCR templates and purification schemes compared to that of the pJL1-sfGFP plasmid. (A) sfGFP yield with different post-PCR purification. The purified PCR template used per reaction was normalized to the same molar concentration of the plasmid pJL1-sfGFP. (B) 15/340 bp upstream: space upstream of T7 promoter; all templates were purified by PCR clean-up. Average sfGFP yield with error bars representing standard deviations of three independent experiments is shown.