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Cell-free colicin production. (A) Total and soluble protein yield of cell-free produced colicins quantified by 14C-Leu scintillation counting. Error bars indicate standard deviations from three independent CFPS samples. (B) Autoradiogram gel of soluble colicins made in CFPS. (C) Time course of soluble colicin Ia production using PCR product (40 ng and 200 ng) and plasmid (200 ng) DNA templates.
