Skip to main content
. 2018 Jun 6;3(1):ysy004. doi: 10.1093/synbio/ysy004

Figure 2.

Figure 2.

Effect of E2 immunity protein on DNA degradation and cell-free E2 production. (A) DNA degradation was assessed by mixing the plasmid DNA pSL101 (1000 ng) with cell-free product containing no colicin (lane 1), colicin E2 (lane 2) and colicin E2 and Immunity protein E2 (lane 3) after incubation at 37°C for 3 h. Purified DNA from these reactions was loaded on a 1% agarose gel. (B) Yield of cell-free synthesized colicin E2 with different molar ratios of E2 immunity protein (E2:Imm = 1:1 or 1:3).