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. 2018 Jun 6;3(1):ysy004. doi: 10.1093/synbio/ysy004

Figure 3.

Figure 3.

Activity of cell-free synthesized colicins. K361 cells were exposed to cell-free synthesized colicins, no added colicin plasmid was assessed as a control (No Col). (A) K361 cells (initial cell density 5 × 106 CFU/ml) where treated with various concentrations of colicin E1 for 60 min at 37°C at 220 rpm. (B) Time course cell viability upon the addition of colicin E1 (750 ng/ml). (C) Cell growth of K361 culture (initial cell density 5 × 107 CFU/ml) with colicin E1 (750 ng/ml) treatment. The inset picture shows K361 culture after a 60 min exposure of cells to E1. Cell-killing activities of colicins Ia, E1 and E2 (750 ng/ml) at (D) low initial cell density (5 × 106 CFU/ml) and (E) high initial cell density (5 × 108 CFU/ml) during growth in LB medium and under a nutrient-free, non-growing condition in 0.85% NaCl solution. Error bars indicate standard deviations from two independent cultures with three plating replicates each. *Represents significant difference compared to No Col samples under the same media conditions with P-value <0.05. ND indicates ‘not detected’.