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. 2017 Dec 8;2(1):ysx006. doi: 10.1093/synbio/ysx006

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — I-FFL schemes. (A) Canonical I-FFL circuits. Composing elements: input (X), auxiliary regulator (Y), and output (Z). (B) Design of a genetic type 1 I-FFL or I1-FFL circuit from BioBricks. Transcription units are numbered 1–4 under the regulation of P_ars, P_luxR, P_ci and P_hyb promoters, respectively. Arsenic (Ars) and mRFP are the input and output signals of the system. To exploit the modular design, the sensor (transcription unit 1) and effector (transcription units 2–4) modules could be located in two different bacteria. Activation is represented by normal arrows and repression by barred arrows.