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. 2017 Dec 8;2(1):ysx006. doi: 10.1093/synbio/ysx006

Figure 3.

Figure 3.

Characterization of constructed iGEM parts. (A) Fluorescence measurements from green fluorescent protein production under the regulation of Pars (Bba_K1106004) after 16 h and stimulated with sodium arsenite (0–1000 ppb). Data are expressed as fluorescence units relative to the culture OD600. Excitation: 475 nm, emission: 515 nm. The inset shows arsenite levels below 100 ppb. (B) AHL determinations in C. violaceum top agar wells inoculated with E. coli cultures. The production of AHL in the culture is evidenced by the violet ring developed. Cultures harboring LuxI under the regulation of Pars (Bba_K1106008) were incubated with 0 or 1000 ppb arsenite for 16 h. Negative control: E coli culture carrying a pSB1C3 empty plasmid. Positive control: R. leguminosarum culture. (C) Fluorescence measurements from mRFP production under the regulation of Pars (Bba_K1106003) after 16 h and stimulated with 25, 50, 100, 200 or 1000 ppb arsenite. Data are expressed as fluorescence units relative to the culture OD600. Excitation: 585 nm, emission: 608 nm. The inset above shows arsenite levels below 100 ppb. (D) Pellets from cultures harboring mRFP under the induction of Pars after incubation with 0, 10, 50, 200 or 1000 ppb arsenite. Aliquots of 1 ml were harvested and centrifuged after 12, 24, 36, 48, 50 or 62 h.