Fig. 1.
Quantification of Aβ40 and Aβ42 levels by sandwich ELISAs in the hippocampus and prefrontal cortex of AppNL-G-F and AppNL mice. a, b AppNL mice do not display changes of soluble Aß40 (a) and Aß42 (b) between 1 and 6 months of age. AppNL-G-F mice present low levels of Aß40 with no age-dependent changes, both in the hippocampus and cortex (a). While the Aβ42 levels in these mice are similar between 1 and 3 months, they increase in the hippocampus but not in the cortex of 6-month-old mice (b). c, d Insoluble Aß40 (c) and Aß42 (d) remain at similar levels in the hippocampus and cortex of AppNL mice across the whole age range. Interestingly, in AppNL-G-F mice, Aß40 is at plateau levels between 1 and 3 months and increases at 6 months, but levels of Aß42 accumulate almost linearly during aging. Of note, the increase in insoluble Aβ42 appears to start earlier in the cortex than in the hippocampus (d). Data represent mean ± SEM (n = 2 mice per indicated time point)