Fig. 4.

Extracellular recordings in the medial PFC of cortical slices from App^NL and App^NL-G-F mice at 3–4 (a, b) and 6–8 months of age (c, d). a Basal synaptic transmission is unaffected at 3–4 months as shown by the input-output curves. b Long-term potentiation induced by 4× HFS in the prelimbic and infralimbic region of the PFC from App^NL-G-F mice is of similar magnitude as from the App^NL littermates, but the potentiation of App^NL-G-F decays faster resulting in a temporary difference to App^NL from 30 min post-induction until 120 min (F_{(1, 7)} = 9.052, p = 0.0197, RM-ANOVA). c Input-output curves indicate a severe reduction in basal synaptic transmission in App^NL-G-F mice at 6–8 months of age (two-way RM-ANOVA F_{(1, 15)} = 16.82, p = 0.0009). d Although LTP of a similar initial magnitude could be induced in App^NL-G-F mice (n = 8), the potentiation was not maintained and declined to baseline. In contrast, App^NL mice (n = 8) expressed a robust LTP that remained at about the same level until the end of recordings resulting in a highly significant genotype difference (RM-ANOVA F_{(1, 16)} = 12.04, p = 0.0032). Data are shown as mean ± SEM. Asterisks indicate difference in fEPSPs between App^NL and App^NL-G-F: *p < 0.05, **p < 0.01, ***p < 0.001 (RM-ANOVA). Insets depict representative analogue traces, taken during baseline recording (solid line), after HFS (long-broken line), 90 min after LTP induction (broken line), and at the end of recording (225 min, dotted line). Note that the analogue traces of App^NL-G-F mice at 90 min and 225 min in b are exactly on top of each other