Table 2.

PCR reaction conditions

Genes	Initial denature	Cycle parameters	Final extension	Cycle number
COXI	94 °C 5 min	94 °C 30 s; 60 °C 30 s; 72 °C 45 s	72 °C 10 min	25
PGC-1α	94 °C 5 min	94 °C 30s; 64 °C 45 s; 72 °C 45 s	72 °C 10 min	30
UCP2	94 °C 5 min	94 °C 30s; 55 °C 30 s; 72 °C 1 min	72 °C 10 min	30