FIGURE 6.
Different stimuli have different effects on microvascular growth in the mouse mesentery. After giving an IP injection of 0.5 mL doses of compound 48–80 (40, 80, 120, 160, and 200 μg/mL in sterile saline) over the time course of 3 d (twice a day and once on the last day) in increasing concentrations to adult, female C57 BL /6 mice (n = 2), PECAM-positive microvessels were not identified after 10 d post-injection (A). PECAM-positive endothelial cells comprising microvessels were observed after 4 wk of injected transformed mouse ovarian epithelial cells (3 × 106 cells in 0.2 mL of PBS ), where spheroid tumors contained microvasculature into the mesenteric window (B). Arrows identify spheroid tumors which are comprised of TARS-positive cells. The solid lines denote the border between the connective tissue and the adipose tissue (+). Scale bars = 100 μm