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. Author manuscript; available in PMC: 2020 Aug 25.
Published in final edited form as: Dev Cell. 2019 Aug 15;50(5):557–572.e5. doi: 10.1016/j.devcel.2019.07.021

Figure 6: Tissue-specific Snz over-expression promotes TAG storage and organismal homeostasis in a DESAT1-dependent manner.

Figure 6:

A) Total larval TAG levels in control (Dcg-Gal4 alone) and Snz transgenic (Tg) larvae with FB-specific over-expression of Snz (Dcg-Gal4>UAS-Snz). * indicates p<0.05. B) Total adult fly TAG from control (Dcg-Gal4) and Snz-Tg flies. * indicates p<0.05. C) FB tissue peripheries of control (Dcg-Gal4) or Snz-Tg and stained for LDs (MDH, magenta). Single cells are outlined in white. Scale bar 10μm. D) Average LD size in the periphery of control and Snz-Tg tissue as in Panel C. * indicates p<0.05. E) Starvation survival assay using 7-10 day old control (Dcg-Gal4) or Snz-Tg flies over-expressing Snz in the FB. F) Total fly TAG levels of control and Snz-Tg flies at time intervals during a starvation assay as in Panel E. * indicates p<0.05. G) Median lifespan of control (Dcg-Gal4) and Snz-Tg flies reared on a normal diet. * indicates p<0.05. H) Fold-enrichment plot for mass spectrometry-based proteomics of Snz-GFP (Dcg-Gal4>UAS-Snz-GFP) and soluble GFP (Dcg-Gal4>UAS-GFP) immuno-precipitates from larval tissue. I) Total fly TAG in control (Dcg-Gal4), Snz-Tg, FB-specific DESAT1RNAi (Dcg-Gal4>UAS-DESAT1RNAi), and DESAT1RNAi + Snz-Tg (Dcg-Gal4>UAS-DESAT1RNAi; Dcg-Gal4>UAS-Snz-GFP). * indicates p<0.05. J) Total larval TAG for 7-10 day old flies for samples as in Panel I. * indicates p<0.05. K) FB peripheries stained for LDs (MDH, magenta) in control (Dcg-Gal4) or DESAT1RNAi samples. Single cells are outlined in white. Scale bar 10μm. L) Relative abundances of # of double bonds in TAG-associated fatty acyl chains from total TAG in control (Dcg-Gal4) and Snz-Tg (Dcg-Gal4>UAS-Snz) larvae. * indicates p<0.05.