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. 2020 Aug 25;20:261. doi: 10.1186/s12906-020-03055-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Immunohistochemical staining of MKK4, p-MKK, ICAM1 and VCAM1 in rat synovium tissue. (A) Control group and (B) model group were treated with saline for 5 days. (C) High dose group (D) middle dose group and (E) Low group were treated with extract of RDN for 5 days, and dose amounted to 40 mg/kg, 80 mg/kg and 160 mg/kg daily, respectively. Each slide was examined at a magnification of 200 times and purple MKK4, p-MKK, ICAM1 and VCAM1 positive cell can be found within rat synovium tissue. Images shown are a representative from three independent trials. ^#p < 0.5 vs control group (independent samples t-test), *p < 0.05 vs model group (one-way ANOVA followed by Dunnett’s t-test)