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. 2020 Aug 25;19:130. doi: 10.1186/s12943-020-01249-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Upregulation of METTL14 enhances the growth and metastasis of pancreatic cancer. a Viability of PANC-1 cells expressing shCtrl, shMETTL14, vector or exogenous METTL14 detected by the CCK8 assay. ***p < 0.001. b Representative images from the colony-forming assay (lower panel) and colony number analysis (upper panel). All experiments were performed in triplicate and data are presented as the mean ± SD. **p < 0.01; ***p < 0.001. c Images (left panel; scale bar: 1 cm) and weight analysis (right panel) of subcutaneous tumors from the indicated groups. * p < 0.05; ** p < 0.01. d Images of the orthotopic transplantation mouse model (shCtrl, shMETTL14, vector or METTL14 groups; lower panel), and analysis of the orthotopic tumor diameter (upper panel). * p < 0.05. PANC-1 cells expressing shCtrl, shMETTL14, vector or METTL14 were subjected to a transwell assay with or without Matrigel (Scale bar: 200 μm) (e), and to a wound-healing assay (Scale bar: 200 μm) (f). All experiments were performed in triplicate and data are presented as the mean ± SD. ** p < 0.01; ***p < 0.001. g Images of armpit lymph node metastasis in the subcutaneous implantation model (left panel) and the respective quantitative analysis (right panel). * p < 0.05. h Statistical analysis of the average number of liver metastases per group in the orthotopic transplantation mouse model. Scale bar: 1 mm. * p < 0.05; ** p < 0.01. i Statistical analysis of the average number of liver metastases per group in the liver metastasis model. ** p < 0.01; ***p < 0.001