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. 2020 Aug 25;19:130. doi: 10.1186/s12943-020-01249-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PERP is the key target of METTL14 in pancreatic cancer. a qPCR analysis of METTL14 and PERP in PANC-1 cells expressing shCtrl or shMETTL14. ***p < 0.001. b Western blotting of METTL14 and PERP in PANC-1 cells expressing shCtrl, shMETTL14, vector or METTL14. c MeRIP-qPCR analysis of fragmented PERP RNA from PANC-1 (control and METTL14 depleted) cells. ** p < 0.01. d qPCR analysis of PERP mRNA levels in PANC-1 cells (control and METTL14 depleted) after actinomycin D treatment. e PANC-1 cells were pre-transfected with wild-type or mutated pmiR-RB-Report-PERP-3′UTR plasmids, and then treated as indicated. Renilla luciferase activity was normalized to firefly luciferase activity and expressed as the mean ± SD. * p < 0.05; ** p < 0.01; ***p < 0.001; n.s., no significance. f Correlation between METTL14 and PERP protein levels in pancreatic cancer specimens. Left - representative IF images of pancreatic cancer specimens. Scale bar, 20 μm. Right - percentage of PERP-positive cells among METTL14-positive versus METTL14-negative cells in selected microscope fields of each tumor (compared by the t-test). * p < 0.05. g qPCR analysis of PERP in PANC-1 cells (control and YTHDF2 depleted). (H) qPCR analysis of PERP in PANC-1 cells (control and YTHDF2 depleted) in the absence or presence of METTL14 overexpression. * p < 0.05; ** p < 0.01. i qPCR analysis of PERP mRNA levels in PANC-1 cells (control and YTHDF2 depleted) in the absence or presence of METTL14 overexpression, and after actinomycin D treatment