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. 2020 Aug 24;17:29. doi: 10.1186/s12950-020-00260-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Validation of microarray transcriptomic results using qPCR. a Box-and-whisker plots for the distribution of the qPCR expression levels (δCq) of the selected genes. The boxes represent the quartiles while the whiskers extend to the most extreme data point which is no more than 1.5 times the interquartile range from the box. The horizontal brackets indicate the statistical significance of the corresponding comparisons (*,**,*** mean p-value smaller than 0.05, 0.01, 0.001, respectively). b Scatter plots comparing the mouse colon differential expression values obtained by microarray (horizontal axis) and qPCR (vertical axis). The following similarity metrics were indicated: “beta” is the slope of the best intercept-free linear fit between microarray and qPCR values, “r2” is the coefficient of determination measuring the “goodness-of-fit”, and “pval” is the p-value associated to the null hypothesis beta = 0. Nic 20, nicotine 20 mg/kg; Ana 20, anatabine 20 mg/kg