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. 2020 Aug 24;19:128. doi: 10.1186/s12943-020-01246-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — circSMARCA5 decreases the expression of SMARCA5 in cells. a Generation of circSMARCA5-knockdown and circSMARCA5-overexpressing cells. MCF-7 cells were infected with lentiviruses expressing shRNA against circSMARCA5 (sh-circSMARCA5; three different oligonucleotides) or circSMARCA5 (pLCDH-circSMARCA5). RT-qPCR was performed to evaluate the expression of circSMARCA5. GAPDH was used as an internal control. b RT-qPCR showing the levels of circSMARCA5 and SMARCA5 in MCF-7 cells stably expressing sh-NC, sh-circSMARCA5, pLCDH-ciR (control), or pLCDH-circSMARCA5. c Western blot showing the levels of SMARCA5 in MCF-7 cells stably expressing sh-NC, sh-circSMARCA5, pLCDH-ciR (control), pLCDH-circSMARCA5, pLCDH-circSMARCA5Δ (without splicing-inducing sequence). GAPDH was used as an internal control. (D-F) The ratio of circ-to-linear of circSMARCA5 in tumor tissue were significantly lower than normal tissue in breast cancer samples (d) and RCC samples (e) (P < 0.05). A negative correlation between circSMARCA5 and SMARCA5 expression was observed in breast cancer samples (d), RCC samples (e), and various cell lines (f)