Fig. 3.

cirSMARCA5 terminates the transcription of SMARCA5 at exon 15. a RT-qPCR analysis of the expression of SMARCA5 in MCF-7 cells using a series of paired primers. “*” indicates P < 0.05. b Rapid amplification of cDNA ends (RACE) PCR analysis of SMARCAC5 transcripts. The PCR products were readily identified by agarose gel electrophoresis. Each set of samples was repeated three times. c Sanger sequencing of two transcripts of SMARCAC5 that are regulated by circSMARCA5 overexpression. d Northern blotting using the junction-specific probes for exons 13-14 and 15-16 to show the expression levels of the transcripts of SMARCAC5 mRNA from MCF-7 cells stably expressing control vector or pLCDH-circSMARCA5 (circ-OE). e CircSMARCA5 prevents transcription from exon 15 of SMARCA5. ChIP-seq analysis showing that the binding of pol II to exons 1-14 of SMARCA5 was higher than that to exons 15-24. ChIP-qPCR showed that the ectopic expression of circSMARCA5 decreased the binding of Pol II to exons 15-24 of SMARCA5