Fig. 8.

Figure 8. circSMARCA5 overexpression enhances the cisplatin response in breast cancer. a MCF-7 cells expressing control vector or pLCDH-circSMARCA5 were transplanted into mice. After the average tumor volume reached 0.3 cm3, the mice were injected with cisplatin or placebo. (Left) Representative images of the isolated tumors from injected mice. (Right) Tumor weight was calculated according to the formula provided in the Materials and Methods section. b After injection with cisplatin or placebo, the mean tumor volume was measured by calipers on the indicated days. c Representative immunofluorescence staining images (scale bars, 100 μm) and SMARCA5 and γH2AX expression in xenograft tumors derived from MCF-7 cells as in A. d RT-qPCR assay showing the relative levels of circSMARCA5 in the tumor tissues and blood of xenograft mice. The blood of tumor-free mice was treated as a control. “*” represents P < 0.05. e Proposed model for circSMARCA5 downregulation of its parent gene expression