Fig. 2.

Dose titration: 10 µg IT dose is the lowest dose which maintains local and systemic anti-tumor efficacy. a Mice were treated with four biweekly doses of the triple combination αCD137, αOX40, and αCTLA4 at two dose levels delivered intratumorally to the left tumor of a dual tumor A20 lymphoma model. Tumor growth measure by caliper of untreated (b), triplet 10 µg (c), or 3 µg (d) injected tumor or non-injected tumor (e–g, respectively). Triplet 10 µg was not significantly different than triplet 30 µg (not shown). Representative photos of the mice at day 19 of tumor inoculation are shown in (h–j).’*’ denotes p < .05 by Student’s t test. n = 5 per group