Fig. 6.

Biodistribution studies: monoclonal antibody (mAb) biodistribution studies, 6 h post-injection, confirm that antibodies concentrate in target structures. a A dual tumor A20 model was used. Mice were injected once with the triple combination (αCD137, αOX40, and αCTLA4) at a dose of 10 µg each. Routes of administration for the different groups are as shown. Mice were sacrificed 6 h post-injection, and tumor, tDLN, non-tDLN, blood, and spleen were collected. b–g Antibody levels in the different compartments were analyzed by ELISA. Values expressed as mean ± SD. n = 3 mice per group per injection site. tDLN tumor draining lymph node; SC subcutaneous. ‘*’ denotes p < .05, Student’s t test. Data for 3 and 24 h time points shown in Supplementary Figure 3