Fig 1.

(A) Enrichment scores (single-sample gene set enrichment analysis) of selected transcriptional signatures across alveolar rhabdomyosarcoma, desmoplastic small round blue cell tumors, Ewing sarcoma, and synovial sarcoma. Desmoplastic small round blue-cell tumor (DSRCT) sarcomas are clearly overenriched in androgen receptor (AR), epidermal growth factor receptor (EGFR), and EWS-WT1 (KTS positive/negative) signatures compared with those of other sarcomas. Variant fusion isoforms of KTS are generated as a result of alternative mRNA splicing. Some of these cases express full-length WT1 or have variant transcripts (KTS positive), which results in atypical staining patterns. KTS-positive variant has transcriptional targets that are different than those of the KTS-negative isoform. Ewing sarcomas are enriched in the EWS-FLI1 signature as expected. (B) Enrichment scores of the AR signature in DSRCTs and three others cancer types—prostate, endometrial, and glioblastoma multiforme (GBM). The highest level of enrichment of the AR signature takes place in prostate cancer. DSRCT sarcomas display increased levels of AR enrichment compared with endometrial and GBM cancers. ARMS, alveolar rhabdomyosarcoma; ES, Ewing sarcoma; SS, synovial sarcoma.