Fig 3.

EGFR V769M mutation activates mitogen-activated protein kinase and AKT signaling. 293T cells were transiently transfected with plasmids that encode wild type (WT) EGFR or EGFR mutants with the following changes: V769M, L858R, and T790M. After 36 hours, cells were serum starved for 24 hours, then harvested for immunoblot analyses (Appendix). Quantification of the protein expression of phospho-epidermal growth factor receptor (pEGFR), total EGFR, phospho-ERK (pERK), total-ERK, phospho-AKT (pAKT), and total AKT was analyzed by using ImageJ. Protein levels relative to cells transfected with WT are expressed as fold changes (mean ± standard deviation; n = 3). (*)P < .05, Student’s t test, each condition compared to WT.