Fig 2.

AR amplification, mutation (L702H), and MYC amplification are associated with resistance to abiraterone. (Left) AR L702H mutation was found in the post-treatment sample in patient Abi-2, but was absent before treatment. Variant allelic frequency in pre- and post-treatment tumors present in genes reported from the COSMIC Cancer Gene Census (Appendix) are shown in the scatter plot. AR L702H missense mutation is circled in red. (Right) AR L702H mutation site was visualized by the Integrated Genomics Viewer (IGV) genome browser. The genomic position is shown at the top of the IGV panel. The variant nucleotide is positioned in the middle. From top to bottom are shown sequencing reads in pretreatment tumor, post-treatment tumor, and germline samples. Colored letters indicate differences from the reference sequence. Change of nucleotide, amino acid, and number of reads in reference and variant alleles are shown in the box. Read strand is shown in pastel colors: red for positive 5′ to 3′ DNA strand, and blue for negative reverse-complement DNA strand.