FIG 3.

Functional assessment of p.R235Q DPYD variant. The in vitro enzyme activity of recombinantly expressed *2A (c.1905+1G>A), *13 (c.1679T>G, p.I560S), p.D949V (c.2846A>T), p.R235Q (c.704G>A), and p.R235W (c.703C>T) variants was compared with reference (wild-type [WT]) dihydropyrimidine dehydrogenase (DPD). For each variant, the mean of 4 independent biologic replicate experiments is presented as a horizontal bar ± standard deviation (SD). Each biologic replicate was measured in triplicate (ie, 3 technical replicates each).