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Patient enrollment and sample collection. Patients with biopsy-proven metastatic prostate cancer treated with androgen receptor (AR)-directed therapy were enrolled in the study and samples were collected for tissue, cell-free DNA (cfDNA), and circulating tumor cell (CTC) analyses. EnhanceAR-Seq, Enhancer and Neighboring Loci of Androgen Receptor Sequencing; FFPE, formalin-fixed paraffin-embedded; NGS, next-generation sequencing.
