FIG A1.

Examples of copy number determination in the androgen receptor (AR) enhancer and gene body from cell-free DNA (cfDNA). Each panel depicts the log₂ copy number ratio of the AR locus and surrounding genomic space, from patient cfDNA normalized to matched plasma-depleted whole blood targeted next-generation sequencing. (A) Example of a patient with no copy number alterations in the AR enhancer or gene body. (B) cfDNA from a patient with copy number gain in the AR gene body but not enhancer. (C) Patient with cfDNA amplification of both the AR enhancer and gene body. (D) Patient with cfDNA copy number gain in the AR enhancer but not gene body. CN, copy number; chrX, chromosome X; log₂, logarithm base 2; Mb, megabase.